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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & focal adhesion sites.
Antibody dilution
1:99
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA057434
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules. Cells in glomeruli were moderately stained.
Expression
RNA: detected in 28 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA057434
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Target mass (kDa)
13.3, 12.8
Antibody dilution
1:330
PROTEIN ARRAY
Antibody HPA057434
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
