ADAMTS9 - Antibodies - The Human Protein Atlas

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ADAMTS9

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA028567

HPA028577

HPA028601

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA028567, RRID:AB_10599275

Atlas Antibodies Cat#HPA028577, RRID:AB_10600330

Atlas Antibodies Cat#HPA028601, RRID:AB_2672694

VALIDATION SUMMARY

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum & vesicles.

Antibody dilution

1:47

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA028577 across 76 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA028567 across 76 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.

Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in paneth cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 53 cell types

RNA: detected in 36 tissues
Protein: detected in 49 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:35

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

216.5, 213.4, 110.2

216.5, 213.4, 55.1

216.5, 213.4, 110.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:10000

1:3000

ANTIGEN INFORMATION

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

VMCVNYSDHVIDRSECDQDYIPETDQDCSMSPCPQRTPDSGLAQHPFQNE
DYRPRSASPSRTHVLGGNQWRTGPWGACSSTC

GDYFIEPLQSMDEQEDEEEQNKPHIIYRRSAPQREPSTGRHACDTSEHKN
RHSKDKKKTRARKWGERINLAGD

KVVCVDDNKNEVHGARCDVSKRPVDRESCSLQPCEYVWITGEWSECSVTC
GKGYKQRLVSCSEIYTGKENYEYSYQTTINCPGTQPPSVHPCYL

Matching transcripts

ADAMTS9-001 - ENSP00000418735 [100%]
ADAMTS9-003 - ENSP00000295903 [100%]
ADAMTS9-006 - ENSP00000417521 [100%]

ADAMTS9-001 - ENSP00000418735 [100%]
ADAMTS9-002 - ENSP00000419217 [100%]
ADAMTS9-003 - ENSP00000295903 [100%]

ADAMTS9-001 - ENSP00000418735 [100%]
ADAMTS9-003 - ENSP00000295903 [100%]
ADAMTS9-006 - ENSP00000417521 [100%]

Other gene match

ANTIGEN VIEW

ADAMTS9-001	ADAMTS9-002	ADAMTS9-003	ADAMTS9-006

RELEVANT PUBLICATIONS

Antibody HPA028567

Antibody HPA028577

Antibody HPA028601

Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture
Peluffo MC et al
Endocrinology 2011;152(10):3963-74

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.