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PBRM1

ANTIBODY INFORMATION
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015629   HPA059373  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA015629, RRID:AB_1855012   Atlas Antibodies Cat#HPA059373, RRID:AB_2683994  

VALIDATION SUMMARY
 
 

Antibody HPA015629

  

Antibody HPA059373

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.  

Antibody dilution

   1:82  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 63 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:35    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 192.9, 189.6, 187.2, 184, 183.9, 183.7, 182.1, 181.1, 168.5, 126.3, 26.4   192.9, 189.6, 187.2, 184, 183.9, 183.7, 182.1, 181.1  

Antibody dilution

 1:250   1:270  

PROTEIN ARRAY
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3300  

ANTIGEN INFORMATION
 
 

Antibody HPA015629

  

Antibody HPA059373

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 146   98  

Antigen sequence

 
KMEEYDDVNLLTADFQLLFNNAKSYYKPDSPEYKAACKLWDLYLRTRNEF
VQKGEADDEDDDEDGQDNQGTVTEGSSPAYLKEILEQLLEAIVVATNPSG
RLISELFQKLPSKVQYPDYYAIIKEPIDLKTIAQRIQNGSYKSIHA
  
PVIQQPTTPMFVAPPPKTQRLLHSEAYLKYIEGLSAESNSISKWDQTLAA
RRRDVHLSKEQESRLPSHWLKSKGAHTTMADALWRLRDLMLRDTLNIR
 

Matching transcripts

 PBRM1-001 - ENSP00000349213 [100%]
PBRM1-002 - ENSP00000378307 [100%]
PBRM1-003 - ENSP00000386593 [100%]
PBRM1-004 - ENSP00000397662 [100%]
PBRM1-005 - ENSP00000386601 [100%]
PBRM1-006 - ENSP00000386643 [100%]
PBRM1-007 - ENSP00000386529 [100%]
PBRM1-008 - ENSP00000296302 [100%]
PBRM1-009 - ENSP00000338302 [100%]
PBRM1-019 - ENSP00000387775 [100%]
PBRM1-018 - ENSP00000409939 [88%]
  PBRM1-001 - ENSP00000349213 [100%]
PBRM1-002 - ENSP00000378307 [100%]
PBRM1-003 - ENSP00000386593 [100%]
PBRM1-005 - ENSP00000386601 [100%]
PBRM1-006 - ENSP00000386643 [100%]
PBRM1-007 - ENSP00000386529 [100%]
PBRM1-008 - ENSP00000296302 [100%]
PBRM1-009 - ENSP00000338302 [100%]
 

Other gene match

    
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Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.