Expression of MFI2 in cancer - Summary - The Human Protein Atlas

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MFI2

TISSUE

CELL

PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary


Level of antibody staining/expression
	High Medium Low Not detected

GENERAL INFORMATION
Gene name	MFI2
Gene description	Antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
Protein class	CD markers Plasma proteins Predicted secreted proteins
Predicted localization	Secreted
Number of transcripts	3
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Cell line enhanced (A-431, SK-MEL-30)
HPA (normal tissue):	Tissue enhanced (parathyroid gland, salivary gland)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in most tissues.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Secreted protein, tissue location of RNA and protein might differ and correlation is complex. Presumed off target binding observed and disregarded.
Reliability score	Approved based on 1 antibody. HPA004880
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (unfavourable).
Renal cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer

Prostate cancer

STAINING SUMMARY

HPA004880

GENE INFORMATION
Gene name	MFI2 (HGNC Symbol)
Synonyms	CD228, FLJ38863, MAP97, MGC4856, MTf, MTF1
Description	Antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 (HGNC Symbol)
Entrez gene summary	The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008]
Chromosome	3
Cytoband	q29
Chromosome location (bp)	196988621 - 197029816
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000163975 (version 83.38)
Entrez gene	4241
UniProt	P08582 (UniProt - Evidence at protein level)
neXtProt	NX_P08582
Antibodypedia	MFI2 antibodies

PROTEIN BROWSER

MFI2-001	MFI2-003	MFI2-005

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MFI2-001 ENSP00000296351 ENST00000296351	P08582 [Direct mapping] Melanotransferrin Q53XS6 [Target identity:100%; Query identity:100%] Antigen p97 (Melanoma associated) identified by monoclonal antibodies 133.2 and 96.5; Antigen p97 (Melanoma associated) identified by monoclonal antibodies 133.2 and 96.5, isoform CRA_b; Melanoma-associated antigen p97, isoform 2 Show all	CD markers MEMSAT3 predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0005887 [integral component of plasma membrane] GO:0009986 [cell surface] GO:0010756 [positive regulation of plasminogen activation] GO:0046658 [anchored component of plasma membrane] GO:0055072 [iron ion homeostasis] GO:0070062 [extracellular exosome] GO:0090091 [positive regulation of extracellular matrix disassembly] GO:0097286 [iron ion import] GO:1900025 [negative regulation of substrate adhesion-dependent cell spreading] Show all	302 aa 32.7 kDa	Yes	0
MFI2-003 ENSP00000296350 ENST00000296350	P08582 [Direct mapping] Melanotransferrin Show all	CD markers MEMSAT3 predicted membrane proteins SCAMPI predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005506 [iron ion binding] GO:0005515 [protein binding] GO:0005576 [extracellular region] GO:0005615 [extracellular space] GO:0005887 [integral component of plasma membrane] GO:0009986 [cell surface] GO:0010756 [positive regulation of plasminogen activation] GO:0046658 [anchored component of plasma membrane] GO:0055072 [iron ion homeostasis] GO:0070062 [extracellular exosome] GO:0090091 [positive regulation of extracellular matrix disassembly] GO:0097286 [iron ion import] GO:1900025 [negative regulation of substrate adhesion-dependent cell spreading] Show all	738 aa 80.2 kDa	Yes	0
MFI2-005 ENSP00000393439 ENST00000439320	C9JVN1 [Direct mapping] Melanotransferrin Show all	MEMSAT3 predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005576 [extracellular region] Show all	181 aa 19.3 kDa	Yes	0

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The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.