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CDK5

ANTIBODY INFORMATION
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Vicgene Biotechnology
  

Product name

 HPA018977   HPA064535   va-5001  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   14   3  

References

 1      

Proper citation

 Atlas Antibodies Cat#HPA018977, RRID:AB_1846424   Atlas Antibodies Cat#HPA064535, RRID:AB_2685280    

VALIDATION SUMMARY
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Approved     Approved  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.    

Antibody dilution

   1:64    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human testis shows distinct positivity in Leydig cells and subsets of cells in the seminiferus ducts.     Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 30 cell types		     RNA: detected in 37 tissues
Protein: detected in 70 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:15     1:20  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.3   33.3, 29.5   33.3, 29.5  

Antibody dilution

 1:250   1:210   1:500  

PROTEIN ARRAY
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:2550    

ANTIGEN INFORMATION
 
 

Antibody HPA018977

  

Antibody HPA064535

  

Antibody CAB008909

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 109   69    

Antigen sequence

 
RVRLDDDDEGVPSSALREICLLKELKHKNIVRLHDVLHSDKKLTLVFEFC
DQDLKKYFDSCNGDLDPEIVKSFLFQLLKGLGFCHSRNVLHRDLKPQNLL
INRNGELKL
  
TEEQWPSMTKLPDYKPYPMYPATTSLVNVVPKLNATGRDLLQNLLKCNPV
QRISAEEALQHPYFSDFCP
  

											 
																				

																		

										
											
Matching transcripts

												
																					CDK5-001 - ENSP00000419782 [100%] 

											 
																						CDK5-001 - ENSP00000419782 [100%] 
CDK5-003 - ENSP00000297518 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDK5-001
CDK5-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA018977

												
										 
																				
											
											
											
Antibody HPA064535

												
										 
																				
											
											
											
Antibody CAB008909

												
										 
																		

																	

										
											
1.

												
																						CDK5 Regulates Paclitaxel Sensitivity in Ovarian Cancer Cells by Modulating AKT Activation, p21Cip1- and p27Kip1-Mediated G1 Cell Cycle Arrest and Apoptosis

	Zhang S et al

	PLoS One 2015;10(7):e0131833

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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