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CXorf58

ANTIBODY INFORMATION
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA031542   HPA031543   HPA031544  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 12   6   12  

References

      

Proper citation

 Atlas Antibodies Cat#HPA031542, RRID:AB_2673927   Atlas Antibodies Cat#HPA031543, RRID:AB_10603789   Atlas Antibodies Cat#HPA031544, RRID:AB_2673929  

VALIDATION SUMMARY
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  
Immunohistochemistry

Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  

Standard validation

 Uncertain   Uncertain   Uncertain  

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in testis.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in testis.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 71 cell types		   RNA: detected in 1 tissues
Protein: detected in 25 cell types		   RNA: detected in 1 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:8   1:75   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 38.9   38.9   38.9  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA031542

  

Antibody HPA031543

  

Antibody HPA031544

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 78   88   75  

Antigen sequence

 
LRIVSEIRGPYLTVQPLYRPYKQQNQVKFLGRRSKQAQMKVEKMRKVYLA
KEKNTSEVTEPKTGPSGTKDNYHLHSIF
  
THEILKKVAPLEAKLIKDPTMQCKIRFRFRGETFPPFIVFKIFLHTDGHG
YKYFSGKNVLMPSSKAVDDACKLMGERKFHRIIMEDER
  
FPKSKVTDIMDVVTMQDYVQYRSFFDEAPAFSGGRNNSWRKLNLENIPRT
MLMYDIVHYSESGVISNRLRNEMKF
 

Matching transcripts

 CXorf58-001 - ENSP00000368511 [100%]
  CXorf58-001 - ENSP00000368511 [100%]
  CXorf58-001 - ENSP00000368511 [100%]
 

Other gene match

      
ANTIGEN VIEW
CXorf58-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.