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ATP7A

ANTIBODY INFORMATION
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA012887   HPA048107  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA012887, RRID:AB_1845205   Atlas Antibodies Cat#HPA048107, RRID:AB_2680266  

VALIDATION SUMMARY
 
 

Antibody HPA012887

  

Antibody HPA048107

  
Immunocytochemistry

Uncertain   Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Standard validation

 Uncertain   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.   Immunofluorescent staining of human cell line HeLa shows localization to the Golgi apparatus.  

Antibody dilution

 1:7   1:65  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells and glial cells.    

Expression

 RNA: detected in 34 tissues
Protein: detected in 69 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 163.4, 154.4   163.4, 154.4, 30.1  

Antibody dilution

 1:250   1:330  

PROTEIN ARRAY
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:2600  

ANTIGEN INFORMATION
 
 

Antibody HPA012887

  

Antibody HPA048107

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 122   82  

Antigen sequence

 
TETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQID
ASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVND
FMTEHERKGRTAVLVAVDDELC
  
LKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKS
GACEDHSMAQAGEVVLKMKVEGMTCHSCTSTI
 

Matching transcripts

 ATP7A-001 - ENSP00000345728 [100%]
ATP7A-201 - ENSP00000343026 [100%]
  ATP7A-001 - ENSP00000345728 [100%]
ATP7A-201 - ENSP00000343026 [100%]
ATP7A-202 - ENSP00000343678 [100%]
 

Other gene match

    
ANTIGEN VIEW
ATP7A-001
ATP7A-201
ATP7A-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.