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PMPCA

ANTIBODY INFORMATION
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021648   HPA063735  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA021648, RRID:AB_1855491   Atlas Antibodies Cat#HPA063735, RRID:AB_2685108  

VALIDATION SUMMARY
 
 

Antibody HPA021648

  

Antibody HPA063735

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported    
Western blot


Validated
   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063735.		  
Validated

Antibody staining overlaps with antibody HPA021648.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.  

Antibody dilution

 1:222   1:53  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 76 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:125    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Formal validation: Genetic
Validated

Downregulation visible in one of two siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 58.3, 44.9    

Loading control

 Total protein image    

Antibody dilution

 1:508    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 58.3, 44.9   58.3, 44.9, 29.5  

Antibody dilution

 1:250   1:125  

PROTEIN ARRAY
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2150  

ANTIGEN INFORMATION
 
 

Antibody HPA021648

  

Antibody HPA063735

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 105   78  

Antigen sequence

 
VEHEHLVDCARKYLLGVQPAWGSAEAVDIDRSVAQYTGGIAKLERDMSNV
SLGPTPIPELTHIMVGLESCSFLEEDFIPFAVLNMMMGGGGSFSAGGPGK
GMFSR
  
YLNVLNRHHWMYNATSYHHSYEDTGLLCIHASADPRQVREMVEIITKEFI
LMGGTVDTVELERAKTQLTSMLMMNLES
 

Matching transcripts

 PMPCA-001 - ENSP00000360782 [100%]
PMPCA-007 - ENSP00000416702 [100%]
  PMPCA-001 - ENSP00000360782 [100%]
PMPCA-003 - ENSP00000408393 [100%]
PMPCA-007 - ENSP00000416702 [100%]
 

Other gene match

    
ANTIGEN VIEW
PMPCA-001
PMPCA-003
PMPCA-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.