R3HCC1L - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

R3HCC1L

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA037367

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA037367

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA037367, RRID:AB_10696930

VALIDATION SUMMARY

Antibody HPA037367

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA037367

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:53

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA037367

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 63 cell types

Retrieval

HIER pH6

Antibody dilution

1:125

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA037367

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Target mass (kDa)

87.9, 86.3

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA037367

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA037367

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ACMMDTTGMSCSDHVTVDSPYVVAVRIADETSINTRSFSKFVGMSADATP
LHVARSGNDTEDFSNPSACSDIYGESISSHFTESTGKLIESLSDCASSL

Matching transcripts

R3HCC1L-001 - ENSP00000298999 [100%]
R3HCC1L-201 - ENSP00000314018 [100%]
R3HCC1L-202 - ENSP00000359616 [100%]
R3HCC1L-203 - ENSP00000483494 [100%]

Other gene match

ANTIGEN VIEW

R3HCC1L-001	R3HCC1L-201	R3HCC1L-202	R3HCC1L-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.