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Antibody staining overlaps with antibody HPA050434.
Validated
Antibody staining overlaps with antibody HPA003417.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line RH-30 shows localization to the Golgi apparatus.
Antibody dilution
1:111
1:7
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003417
Antibody HPA050434
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts and leydig cells.
Expression
RNA: detected in 37 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
Antibody dilution
1:50
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003417
Antibody HPA050434
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403197)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35
35
Antibody dilution
1:250
1:80
PROTEIN ARRAY
Antibody HPA003417
Antibody HPA050434
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
