Fields »
ABOUT
HELP
BLOG
RPL27A

ANTIBODY INFORMATION
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA060776   HPA063379  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

Proper citation

 Atlas Antibodies Cat#HPA060776, RRID:AB_2684359   Atlas Antibodies Cat#HPA063379, RRID:AB_2684997  

VALIDATION SUMMARY
 
 

Antibody HPA060776

  

Antibody HPA063379

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063379.		  
Validated

Antibody staining overlaps with antibody HPA060776.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to cytosol & endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & endoplasmic reticulum.  

Antibody dilution

 1:20   1:56  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA063379 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA060776 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 46 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:250  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 16.6, 12.2   16.6, 12.5, 12.2, 4.6  

Antibody dilution

 1:80   1:190  

PROTEIN ARRAY
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:550   1:2250  

ANTIGEN INFORMATION
 
 

Antibody HPA060776

  

Antibody HPA063379

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   27  

Antigen sequence

 
INFDKYHPGYFGKVGMKHYHLKRNQSFCPTVNLDKLWTLVSEQTRVNAAK
NKTGAAPIIDVVRSGYYKVLG
  
PSRLRKTRKLRGHVSHGHGRIGKHRKH
 

Matching transcripts

 RPL27A-001 - ENSP00000346015 [100%]
RPL27A-004 - ENSP00000433259 [90%]
  RPL27A-001 - ENSP00000346015 [100%]
RPL27A-004 - ENSP00000433259 [100%]
RPL27A-006 - ENSP00000435081 [100%]
RPL27A-010 - ENSP00000437118 [81%]
 

Other gene match

    
ANTIGEN VIEW
RPL27A-001
RPL27A-004
RPL27A-006
RPL27A-010
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.