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Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm.
Antibody dilution
1:35
1:40
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA048321
Antibody HPA054203
Antibody CAB003843
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human pancreas shows weak nuclear positivity in exocrine glandular cells and weak cytoplasmic in islets of Langerhans.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in cells outside reaction centra.
Expression
RNA: detected in 30 tissues Protein: detected in 47 cell types
RNA: detected in 30 tissues Protein: detected in 37 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048321
Antibody HPA054203
Antibody CAB003843
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
76.9
77, 76.9
77, 76.9, 14.9, 14, 13.8, 6.4
Antibody dilution
1:170
1:200
1:500
PROTEIN ARRAY
Antibody HPA048321
Antibody HPA054203
Antibody CAB003843
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
