Expression of ANPEP in cancer - Summary

GENERAL INFORMATION
Gene name	ANPEP
Gene description	Alanyl (membrane) aminopeptidase
Protein class	CD markers Enzymes Plasma proteins Predicted membrane proteins
Predicted localization	Membrane
Number of transcripts	3
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Cell line enhanced (BJ, BJ hTERT+, HMC-1, HUVEC TERT2, TIME, U-2197)
HPA (normal tissue):	Group enriched (duodenum, small intestine)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Membranous and cytoplasmic expression in renal tubules, intestine, exocrine pancreas, prostate, liver and gallbladder.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data.
Reliability score	Supported based on 2 antibodies. HPA004625 , CAB002417
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PROGNOSTIC SUMMARY
Gene product is not prognostic.
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RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA004625	CAB002417

GENE INFORMATION
Gene name	ANPEP (HGNC Symbol)
Synonyms	CD13, gp150, LAP1, p150, PEPN
Description	Alanyl (membrane) aminopeptidase (HGNC Symbol)
Entrez gene summary	Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Its function in proximal tubular epithelial cells and other cell types is less clear. The large extracellular carboxyterminal domain contains a pentapeptide consensus sequence characteristic of members of the zinc-binding metalloproteinase superfamily. Sequence comparisons with known enzymes of this class showed that CD13 and aminopeptidase N are identical. The latter enzyme was thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Human aminopeptidase N is a receptor for one strain of human coronavirus that is an important cause of upper respiratory tract infections. Defects in this gene appear to be a cause of various types of leukemia or lymphoma. [provided by RefSeq, Jul 2008]
Chromosome	15
Cytoband	q26.1
Chromosome location (bp)	89784889 - 89815401
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000166825 (version 83.38)
Entrez gene	290
UniProt	P15144 (UniProt - Evidence at protein level)
neXtProt	NX_P15144
Antibodypedia	ANPEP antibodies

PROTEIN BROWSER

ANPEP-001	ANPEP-002	ANPEP-003

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
ANPEP-001 ENSP00000300060 ENST00000300060	P15144 [Direct mapping] Aminopeptidase N A0A024RC61 [Target identity:100%; Query identity:100%] Alanyl (Membrane) aminopeptidase (Aminopeptidase N, aminopeptidase M, microsomal aminopeptidase, CD13, p150), isoform CRA_a Show all	Enzymes ENZYME proteins Hydrolases Peptidases Metallopeptidases CD markers Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0001525 [angiogenesis] GO:0001618 [virus receptor activity] GO:0002003 [angiotensin maturation] GO:0004177 [aminopeptidase activity] GO:0004872 [receptor activity] GO:0005615 [extracellular space] GO:0005765 [lysosomal membrane] GO:0005793 [endoplasmic reticulum-Golgi intermediate compartment] GO:0005829 [cytosol] GO:0005887 [integral component of plasma membrane] GO:0006508 [proteolysis] GO:0006725 [cellular aromatic compound metabolic process] GO:0008237 [metallopeptidase activity] GO:0008270 [zinc ion binding] GO:0009897 [external side of plasma membrane] GO:0012506 [vesicle membrane] GO:0030154 [cell differentiation] GO:0031983 [vesicle lumen] GO:0035814 [negative regulation of renal sodium excretion] GO:0042277 [peptide binding] GO:0043171 [peptide catabolic process] GO:0044267 [cellular protein metabolic process] GO:0046718 [viral entry into host cell] GO:0070006 [metalloaminopeptidase activity] GO:0070062 [extracellular exosome] Show all	967 aa 109.5 kDa	No	1
ANPEP-002 ENSP00000452934 ENST00000559874	H0YKT6 [Direct mapping] Aminopeptidase N Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0006508 [proteolysis] GO:0016021 [integral component of membrane] Show all	103 aa 11.1 kDa	No	1
ANPEP-003 ENSP00000453413 ENST00000560137	H0YM04 [Direct mapping] Aminopeptidase N Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0006508 [proteolysis] GO:0016021 [integral component of membrane] Show all	61 aa 6.1 kDa	No	1