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C15orf43

ANTIBODY INFORMATION
 
 

Antibody HPA041780

  

Antibody HPA041941

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041780   HPA041941  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

Proper citation

    

VALIDATION SUMMARY
 
 

Antibody HPA041780

  

Antibody HPA041941

  
Immunocytochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041780

  

Antibody HPA041941

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.   Immunofluorescent staining of human cell line A-431 shows localization to centrosome.  

Antibody dilution

 1:99   1:96  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA041780

  

Antibody HPA041941

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 25.3   25.3  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041780

  

Antibody HPA041941

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041780

  

Antibody HPA041941

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 101   92  

Antigen sequence

 
IEKHDEVTPNEIKTLRENSELATEHKKELSKSPEKHFIRTPVVEKQMYFP
LQNYPVNNMVTGYISIDAMKKFLGELHDFIPGTSGYLAYHVQNEINMSAI
K
  
QGQRGWFCGSVSQDLRQFWVAEGGTISDPRAADFLFSCDASHPDTLRIYQ
SLDYIEDNATVFHAYYLSAVANAKIKNSVALGHFILPPACLQ
 

Matching transcripts

 C15orf43-001 - ENSP00000340644 [100%]
  C15orf43-001 - ENSP00000340644 [100%]
 

Other gene match

    
ANTIGEN VIEW
C15orf43-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.