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CTNNB1

ANTIBODY INFORMATION
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Chemicon
   Upstate
  

Product name

 HPA029159   HPA029160   AB19022   05-665  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known  

Other gene match

        

Released in version

 6   6   1   1  

References

 1        

Proper citation

 Atlas Antibodies Cat#HPA029159, RRID:AB_10599619   Atlas Antibodies Cat#HPA029160, RRID:AB_10600996      

VALIDATION SUMMARY
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  
Immunocytochemistry


Validated
  

Validated
   Supported    
Immunohistochemistry


Validated
  

Validated

Validated
   Supported   Supported  
Western blot

Supported   Uncertain   Supported   Uncertain  
Protein array

Supported   Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA029160.		  
Validated

Antibody staining overlaps with antibody HPA029159.		      
 

Standard validation

 Supported   Supported   Supported    

       

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.    

Antibody dilution

 1:100   1:50   1:19    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

 
     

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 43 cell lines.      
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA029160 across 45 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA029159 across 45 cell lines.		      
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		      

 
     

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA029160 across 72 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA029159 across 72 cell types.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human liver shows strong positivity of cytoplasm and nuclei in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		   RNA: detected in 37 tissues
Protein: detected in 59 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:250   1:75   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 85.5, 84.8, 16.6, 12, 11.1   85.5, 84.8   85.5, 84.8, 16.6, 12, 11.1, 6.5, 1.4   85.5, 84.8, 16.6, 12, 11.1, 6.5, 1.4  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:3000      

ANTIGEN INFORMATION
 
 

Antibody HPA029159

  

Antibody HPA029160

  

Antibody CAB000108

  

Antibody CAB001950

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Not known  

Length (aa)

 90   93      

Antigen sequence

 
SYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQ
VADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAH
  
SEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQD
DPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPD
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CTNNB1-001 - ENSP00000344456 [100%] 
CTNNB1-002 - ENSP00000379488 [100%] 
CTNNB1-003 - ENSP00000379486 [100%] 
CTNNB1-005 - ENSP00000411226 [100%] 
CTNNB1-007 - ENSP00000385604 [100%] 
CTNNB1-012 - ENSP00000412219 [100%] 
CTNNB1-013 - ENSP00000409302 [89%] 
CTNNB1-014 - ENSP00000401599 [80%] 

											 
																						CTNNB1-001 - ENSP00000344456 [100%] 
CTNNB1-002 - ENSP00000379488 [100%] 
CTNNB1-003 - ENSP00000379486 [100%] 
CTNNB1-005 - ENSP00000411226 [100%] 
CTNNB1-007 - ENSP00000385604 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CTNNB1-001
CTNNB1-002
CTNNB1-003
CTNNB1-005
CTNNB1-007
CTNNB1-012
CTNNB1-013
CTNNB1-014
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA029159

												
										 
																				
											
											
											
Antibody HPA029160

												
										 
																				
											
											
											
Antibody CAB000108

												
										 
																				
											
											
											
Antibody CAB001950

												
										 
																		

																	

										
											
1.

												
																						The Urotensin II System and Carotid Atherosclerosis: A Role in Vascular Calcification

	Albanese I et al

	Front Pharmacol 2016;7:149

			
											 
																						
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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