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Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in tubules.
Expression
RNA: detected in 3 tissues Protein: detected in 65 cell types
RNA: detected in 3 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:30
1:30
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045909
Antibody HPA046724
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412657)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412657)
Target mass (kDa)
95.7, 95.6, 94.6
95.7, 95.6, 94.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA045909
Antibody HPA046724
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
