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ANTIBODY INFORMATION
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
   GeneTex
  

Product name

 HPA065701   M3562   GTX62599  

Host species

 Rabbit   Mouse   Rabbit  

Clonality

 pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Supernatant   Supernatant  

Other gene match

      

Released in version

 16   1   14  

References

      

Proper citation

 Atlas Antibodies Cat#HPA065701, RRID:AB_2685535   Dako Cat#M3562, RRID:AB_2060174   GeneTex Cat#GTX62599, RRID:AB_10620739  

VALIDATION SUMMARY
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  
Immunocytochemistry

Approved      
Immunohistochemistry

 

Validated
  

Validated
  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Standard validation

 Approved      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.      

Antibody dilution

 1:143      

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Formal validation: Orthogonal

  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 
   

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.    
 

Formal validation: Orthogonal

  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 
 
 

Figure description

   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunohistochemical staining of human epididymis shows nuclear positivity in glandular cells.   Immunohistochemical staining of human testis shows strong nuclear positivity in Leydig cells and subset of cells in seminiferous duct.  

Expression

   RNA: detected in 24 tissues
Protein: detected in 13 cell types		   RNA: detected in 24 tissues
Protein: detected in 15 cell types		  

Retrieval

   HIER pH9   HIER pH6  

Antibody dilution

   1:25   1:500  

Literature conformity

   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 99.2, 79.4, 77, 67.4, 67.1, 59.4   99.2, 79.4, 77, 67.4, 67.1, 59.4, 44.6   99.2, 79.4, 77, 67.4, 67.1, 59.4, 44.6  

Antibody dilution

 1:322   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:5700      

ANTIGEN INFORMATION
 
 

Antibody HPA065701

  

Antibody CAB000001

  

Antibody CAB065764

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Synthetic peptide  

Length (aa)

 66      

Antigen sequence

 
LSPGEQLRGDCMYAPLLGVPPAVRPTPCAPLAECKGSLLDDSAGKSTEDT
AEYSPFKGGYTKGLEG
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					AR-001 - ENSP00000363822 [100%] 
AR-004 - ENSP00000421155 [100%] 
AR-201 - ENSP00000379359 [100%] 
AR-202 - ENSP00000482407 [100%] 
AR-203 - ENSP00000484033 [100%] 
AR-204 - ENSP00000479013 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AR-001
AR-004
AR-201
AR-202
AR-203
AR-204
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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