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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA054721 across 44 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA043420 across 44 cell lines.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.
Expression
RNA: detected in 5 tissues Protein: detected in 6 cell types
RNA: detected in 5 tissues Protein: detected in 1 cell types
RNA: detected in 5 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:75
1:400
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043420
Antibody HPA054721
Antibody CAB009446
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 107, 78, 46, 31, 26, 16.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
