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DFFB

GENERAL INFORMATION

Gene name

 DFFB

Gene description

 DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Mixed
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Cytoplasmic and nuclear expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Approved based on 1 antibody.
CAB004328
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PROGNOSTIC SUMMARY
Prognostic marker in urothelial cancer (favourable).
Urothelial cancer p<0.001
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
CAB004328

GENE INFORMATION

Gene name

 DFFB (HGNC Symbol)

Synonyms

 CAD, CPAN, DFF-40, DFF40

Description

 DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (HGNC Symbol)

Entrez gene summary

 Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]

Chromosome

 1

Cytoband

 p36.32

Chromosome location (bp)

 3857267 - 3885429

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000169598 (version 83.38)

Entrez gene

 1677

UniProt

 O76075 (UniProt - Evidence at protein level)

neXtProt

 NX_O76075

Antibodypedia

 DFFB antibodies

PROTEIN BROWSER
DFFB-001
DFFB-201
DFFB-202
DFFB-203

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
DFFB-001
ENSP00000367454
ENST00000378209
O76075 [Direct mapping]
DNA fragmentation factor subunit beta
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Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
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GO:0000790 [nuclear chromatin]
GO:0004536 [deoxyribonuclease activity]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005730 [nucleolus]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006309 [apoptotic DNA fragmentation]
GO:0006915 [apoptotic process]
GO:0006921 [cellular component disassembly involved in execution phase of apoptosis]
GO:0012501 [programmed cell death]
GO:0016787 [hydrolase activity]
GO:0019899 [enzyme binding]
GO:0030263 [apoptotic chromosome condensation]
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 338 aa
39.1 kDa 		No 0
DFFB-201
ENSP00000339524
ENST00000338895
B4DZS0 [Direct mapping]
DNA fragmentation factor subunit beta; cDNA FLJ50316, highly similar to DNAation factor subunit beta (EC 3.-.-.-)
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   SCAMPI predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0003677 [DNA binding]
GO:0004518 [nuclease activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0006309 [apoptotic DNA fragmentation]
GO:0006915 [apoptotic process]
GO:0016787 [hydrolase activity]
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 362 aa
42 kDa 		No 0
DFFB-202
ENSP00000345906
ENST00000341385
A0A0B4J1S5 [Direct mapping]
DNA fragmentation factor subunit beta
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005622 [intracellular]
GO:0006915 [apoptotic process]
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 82 aa
9.3 kDa 		No 0
DFFB-203
ENSP00000486916
ENST00000625756
G3XAD0 [Direct mapping]
DNA fragmentation factor subunit beta; DNAation factor, 40kDa, beta polypeptide (Caspase-activated DNase), isoform CRA_a
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Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
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GO:0005622 [intracellular]
GO:0006915 [apoptotic process]
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 103 aa
11.5 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.