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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:75
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA029396
Antibody HPA034642
Antibody HPA034643
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human esophagus shows nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human liver shows distinct cytoplasmic positivity in hepatocytes and bile duct cells.
Expression
RNA: detected in 30 tissues Protein: detected in 69 cell types
RNA: detected in 30 tissues Protein: detected in 79 cell types
RNA: detected in 30 tissues Protein: detected in 45 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:60
1:500
1:40
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA029396
Antibody HPA034642
Antibody HPA034643
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403372)
Target mass (kDa)
44.8
57
57
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA029396
Antibody HPA034642
Antibody HPA034643
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
