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SIK2

ANTIBODY INFORMATION
 
 

Antibody HPA016026

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA016026  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 4  

References

  

Proper citation

 Atlas Antibodies Cat#HPA016026, RRID:AB_1857321  

VALIDATION SUMMARY
 
 

Antibody HPA016026

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA016026

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.  

Antibody dilution

 1:65  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA016026

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human skeletal muscle shows cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA016026

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

 

Figure description

  

Target mass (kDa)

 103.9  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA016026

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA016026

  

Antigen

 Recombinant protein fragment  

Length (aa)

 133  

Antigen sequence

 
HFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLP
VTMHSPNMRLLRSALLPQASNVEAFSFPASGCQAEAAFMEEECVDTPKVN
GCLLDPVPPVLVRKGCQSLPSNMMETSIDEGLE
 

Matching transcripts

 SIK2-001 - ENSP00000305976 [100%]
 

Other gene match

  
ANTIGEN VIEW
SIK2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.