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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

ALK

Gene description

Anaplastic lymphoma receptor tyrosine kinase

Protein class

Cancer-related genes
CD markers
Disease related genes
Enzymes
FDA approved drug targets
Plasma proteins
Predicted intracellular proteins
Predicted membrane proteins

Predicted localization

Intracellular,Membrane

Number of transcripts

4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Group enriched (glioma, melanoma, ovarian cancer, thyroid cancer)
HPA (cell line):Cell line enriched (SH-SY5Y)
HPA (normal tissue):Tissue enhanced (cerebral cortex, testis)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and nuclear expression in several different tissue types, most abundant in neurons.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data. Presumed off target binding observed and disregarded.

Reliability score

Approved based on 1 antibody.
HPA010694
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PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Group enriched (glioma, melanoma, ovarian cancer, thyroid cancer)

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Skin cancer

STAINING SUMMARY

HPA010694

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic staining with a granular pattern was observed in basal cell skin carcinomas, the majority of prostate cancers and a few gliomas and melanomas. Remaining cancer tissues displayed weak cytoplasmic staining or were negative.

GENE INFORMATION

Gene name

ALK (HGNC Symbol)

Synonyms

CD246

Description

Anaplastic lymphoma receptor tyrosine kinase (HGNC Symbol)

Entrez gene summary

This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Chromosome

2

Cytoband

Chromosome location (bp)

29192774 - 29921566

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000171094 (version 83.38)

Entrez gene

238

UniProt

Q9UM73 (UniProt - Evidence at protein level)

neXtProt

NX_Q9UM73

Antibodypedia

ALK antibodies

PROTEIN BROWSER

ALK-001
ALK-002
ALK-201
ALK-202

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ALK-001
ENSP00000373700
ENST00000389048
Q9UM73 [Direct mapping]
ALK tyrosine kinase receptor
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Enzymes
   ENZYME proteins
   Transferases
   Kinases
   Tyr protein kinases
CD markers
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Cancer-related genes
   Mutated cancer genes
   COSMIC somatic mutations in cancer genes
   COSMIC Amplifications
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Translocations
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000187 [activation of MAPK activity]
GO:0004672 [protein kinase activity]
GO:0004704 [NF-kappaB-inducing kinase activity]
GO:0004713 [protein tyrosine kinase activity]
GO:0004714 [transmembrane receptor protein tyrosine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005622 [intracellular]
GO:0005887 [integral component of plasma membrane]
GO:0006468 [protein phosphorylation]
GO:0007165 [signal transduction]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0007399 [nervous system development]
GO:0008283 [cell proliferation]
GO:0016020 [membrane]
GO:0016310 [phosphorylation]
GO:0018108 [peptidyl-tyrosine phosphorylation]
GO:0038061 [NIK/NF-kappaB signaling]
GO:0042802 [identical protein binding]
GO:0042981 [regulation of apoptotic process]
GO:0043234 [protein complex]
GO:0046777 [protein autophosphorylation]
GO:0048666 [neuron development]
GO:0051092 [positive regulation of NF-kappaB transcription factor activity]
GO:0070062 [extracellular exosome]
Show all
1620 aa
176.4 kDa
Yes 1
ALK-002
ENSP00000387488
ENST00000453137
H7BZ33 [Direct mapping]
ALK tyrosine kinase receptor
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Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
   COSMIC somatic mutations in cancer genes
   COSMIC Amplifications
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004672 [protein kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
Show all
152 aa
16.9 kDa
No 0
ALK-201
ENSP00000414027
ENST00000431873
E7EPW7 [Direct mapping]
Tyrosine-protein kinase receptor
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   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
   COSMIC somatic mutations in cancer genes
   COSMIC Amplifications
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000187 [activation of MAPK activity]
GO:0004672 [protein kinase activity]
GO:0004713 [protein tyrosine kinase activity]
GO:0004714 [transmembrane receptor protein tyrosine kinase activity]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0018108 [peptidyl-tyrosine phosphorylation]
GO:0048666 [neuron development]
Show all
580 aa
64.4 kDa
No 0
ALK-202
ENSP00000482733
ENST00000618119
A0A087WZL3 [Direct mapping]
Tyrosine-protein kinase receptor
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Cancer-related genes
   Mutated cancer genes
   COSMIC somatic mutations in cancer genes
   COSMIC Amplifications
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
   COSMIC Germline Mutations
   COSMIC Translocations
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000187 [activation of MAPK activity]
GO:0004672 [protein kinase activity]
GO:0004713 [protein tyrosine kinase activity]
GO:0004714 [transmembrane receptor protein tyrosine kinase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0006468 [protein phosphorylation]
GO:0007169 [transmembrane receptor protein tyrosine kinase signaling pathway]
GO:0016020 [membrane]
GO:0016021 [integral component of membrane]
GO:0018108 [peptidyl-tyrosine phosphorylation]
GO:0048666 [neuron development]
Show all
1243 aa
135 kDa
No 1

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