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ANTIBODY INFORMATION
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Upstate
  

Product name

 HPA044496   HPA054252   05-625  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 13   16   1  

References

      

Proper citation

 Atlas Antibodies Cat#HPA044496, RRID:AB_2678959   Atlas Antibodies Cat#HPA054252, RRID:AB_2682428    

VALIDATION SUMMARY
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.    

Antibody dilution

   1:67    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuropil and neuronal cells.     Immunohistochemical staining of human cerebral cortex shows strong cytopasmic positivity in neuropil.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 18 cell types		     RNA: detected in 36 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:4000     1:30000  

Literature conformity

 Consistent with extensive gene/protein characterization data.     Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 83.7   83.7, 23.9   83.7, 23.9, 14.4  

Antibody dilution

 1:250   1:330   1:500  

PROTEIN ARRAY
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:30000   1:2650    

ANTIGEN INFORMATION
 
 

Antibody HPA044496

  

Antibody HPA054252

  

Antibody CAB001948

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 55   65    

Antigen sequence

 
PPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFG
EDLMP
  
PSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLM
SPGENGEVRQGQAKR
  

											 
																				

																		

										
											
Matching transcripts

												
																					PRKCE-001 - ENSP00000306124 [100%] 

											 
																						PRKCE-001 - ENSP00000306124 [100%] 
PRKCE-008 - ENSP00000378341 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PRKCE-001
PRKCE-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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