Fields »
ABOUT
HELP
BLOG
JUNB

ANTIBODY INFORMATION
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA019149   sc-8051  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 5   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA019149, RRID:AB_1852053   Santa Cruz Biotechnology Cat#sc-8051, RRID:AB_2130023  

VALIDATION SUMMARY
 
 

Antibody HPA019149

  

Antibody CAB004464

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.    

Antibody dilution

 1:154    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.   Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 33 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:75  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 35.9   35.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA019149

  

Antibody CAB004464

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 119    

Antigen sequence

 
SLHDYKLLKPSLAVNLADPYRSLKAPGARGPGPEGGGGGSYFSGQGSDTG
ASLKLASSELERLIVPNSNGVITTTPTPPGQYFYPRGGGSGGGAGGAGGG
VTEEQEGFADGFVKALDDL
  

											 
																				

																		

										
											
Matching transcripts

												
																					JUNB-001 - ENSP00000303315 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
JUNB-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.