AGXT - Antibodies - The Human Protein Atlas

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AGXT

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA035370

Antibody HPA035371

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035370

HPA035371

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA035370, RRID:AB_10673218

Atlas Antibodies Cat#HPA035371, RRID:AB_2674593

VALIDATION SUMMARY

Antibody HPA035370

Antibody HPA035371

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035370

Antibody HPA035371

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.

Antibody dilution

1:127

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA035370

Antibody HPA035371

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in granular pattern in hepatocytes.

Immunohistochemical staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 2 tissues
Protein: detected in 5 cell types

RNA: detected in 2 tissues
Protein: detected in 17 cell types

Retrieval

HIER pH6

Antibody dilution

1:1500

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035370

Antibody HPA035371

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA035370

Antibody HPA035371

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA035370

Antibody HPA035371

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

HPMTKDPGGHYTLQEVEEGLAQHKPVLLFLTHGESSTGVLQPLDGFGELC
HRYKCLLLVDSVASLGGTPLYMDRQGIDILYSGS

QLFVKDPALRLPTVTTVAVPAGYDWRDIVSYVIDHFDIEIMGGLGPSTGK
VLRIGLLGCNATRENVDRVTEALRAALQHCPKKKL

Matching transcripts

AGXT-001 - ENSP00000302620 [100%]

Other gene match

ANTIGEN VIEW

AGXT-001

RELEVANT PUBLICATIONS

Antibody HPA035370

Antibody HPA035371

Helper-dependent adenoviral vectors for liver-directed gene therapy of primary hyperoxaluria type 1
Castello R et al
Gene Ther 2016;23(2):129-34

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.