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ANTIBODY INFORMATION
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA063958   HPA067638   HPA070981  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 14   14   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA063958, RRID:AB_2685160   Atlas Antibodies Cat#HPA067638, RRID:AB_2685876   Atlas Antibodies Cat#HPA070981, RRID:AB_2686331  

VALIDATION SUMMARY
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Approved   Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA067638.		  
Validated

Antibody staining overlaps with antibody HPA063958.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.  

Antibody dilution

 1:35   1:200   1:102  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

WESTERN BLOT
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 92.5   92.5   92.5  

Antibody dilution

 1:80   1:930   1:250  

PROTEIN ARRAY
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1250   1:11200   1:4050  

ANTIGEN INFORMATION
 
 

Antibody HPA063958

  

Antibody HPA067638

  

Antibody HPA070981

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 70   76   69  

Antigen sequence

 
IQELTIEEHLIERKKKLQEKKMHIAALASAILSDPENNIKKLKELRSMLM
EQDPDVAVTVRKLVIVSLME
  
DDLQLMKDLGQRVSFLTRDLSSSEPVHAKKRKHERIIDKYEKIPRTLQTA
PEKELIHLLPIKDKSGIIPQTREKPV
  
GSGALKPELSRRSATELFEAYSMAEMTFNPPVESSNPKIKGKFLQGDSFL
NEDLNQLIKRYSSEVATES
 

Matching transcripts

 NOC3L-001 - ENSP00000360412 [100%]
  NOC3L-001 - ENSP00000360412 [100%]
  NOC3L-001 - ENSP00000360412 [100%]
 

Other gene match

      
ANTIGEN VIEW
NOC3L-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.