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C2orf70

ANTIBODY INFORMATION
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA062453   HPA069279  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA062453, RRID:AB_2684769   Atlas Antibodies Cat#HPA069279, RRID:AB_2686106  

VALIDATION SUMMARY
 
 

Antibody HPA062453

  

Antibody HPA069279

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A549 shows localization to nucleus, cytosol & vesicles.  

Antibody dilution

   1:36  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate nuclear membranous positivity in neuronal cells    

Expression

 RNA: detected in 4 tissues
Protein: detected in 26 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:400    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 23.4   23.4  

Antibody dilution

 1:240   1:120  

PROTEIN ARRAY
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2800   1:1300  

ANTIGEN INFORMATION
 
 

Antibody HPA062453

  

Antibody HPA069279

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 64   62  

Antigen sequence

 
SAGTLLTEFNAAYVPPGLMPGYQGHVPTVAFSFGAPYGTTTLKYFQDHRN
RAMEKSHTPFSQGG
  
PTIFSTNPNLLLMERASTRDRWLHKPSYTRFNLDSHRSTELTNFYQMVQQ
HRKYYQDKTGTV
 

Matching transcripts

 C2orf70-004 - ENSP00000332875 [100%]
  C2orf70-004 - ENSP00000332875 [100%]
 

Other gene match

    
ANTIGEN VIEW
C2orf70-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.