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PRPF8

GENERAL INFORMATION

Gene name

 PRPF8

Gene description

 Pre-mRNA processing factor 8

Protein class

 Cancer-related genes
Disease related genes
Plasma proteins
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Ubiquitous nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Supported based on 2 antibodies.
CAB009941 , CAB015457
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PROGNOSTIC SUMMARY
Gene product is not prognostic.
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer

STAINING SUMMARY
CAB009941
CAB015457

GENE INFORMATION

Gene name

 PRPF8 (HGNC Symbol)

Synonyms

 hPrp8, Prp8, PRPC8, RP13, SNRNP220

Description

 Pre-mRNA processing factor 8 (HGNC Symbol)

Entrez gene summary

 Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]

Chromosome

 17

Cytoband

 p13.3

Chromosome location (bp)

 1650629 - 1684882

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000174231 (version 83.38)

Entrez gene

 10594

UniProt

 Q6P2Q9 (UniProt - Evidence at protein level)

neXtProt

 NX_Q6P2Q9

Antibodypedia

 PRPF8 antibodies

PROTEIN BROWSER
PRPF8-001
PRPF8-002
PRPF8-003
PRPF8-013
PRPF8-017

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
PRPF8-001
ENSP00000304350
ENST00000304992
Q6P2Q9 [Direct mapping]
Pre-mRNA-processing-splicing factor 8
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   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutational cancer driver genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000244 [spliceosomal tri-snRNP complex assembly]
GO:0000375 [RNA splicing, via transesterification reactions]
GO:0000386 [second spliceosomal transesterification activity]
GO:0000398 [mRNA splicing, via spliceosome]
GO:0003676 [nucleic acid binding]
GO:0003723 [RNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005682 [U5 snRNP]
GO:0006397 [mRNA processing]
GO:0008380 [RNA splicing]
GO:0010467 [gene expression]
GO:0016020 [membrane]
GO:0016607 [nuclear speck]
GO:0017070 [U6 snRNA binding]
GO:0030619 [U1 snRNA binding]
GO:0030620 [U2 snRNA binding]
GO:0030623 [U5 snRNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0070530 [K63-linked polyubiquitin binding]
GO:0071013 [catalytic step 2 spliceosome]
GO:0097157 [pre-mRNA intronic binding]
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 2335 aa
273.6 kDa 		No 0
PRPF8-002
ENSP00000460348
ENST00000572621
Q6P2Q9 [Direct mapping]
Pre-mRNA-processing-splicing factor 8
Show all
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutational cancer driver genes
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000244 [spliceosomal tri-snRNP complex assembly]
GO:0000375 [RNA splicing, via transesterification reactions]
GO:0000386 [second spliceosomal transesterification activity]
GO:0000398 [mRNA splicing, via spliceosome]
GO:0003676 [nucleic acid binding]
GO:0003723 [RNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005682 [U5 snRNP]
GO:0006397 [mRNA processing]
GO:0008380 [RNA splicing]
GO:0010467 [gene expression]
GO:0016020 [membrane]
GO:0016607 [nuclear speck]
GO:0017070 [U6 snRNA binding]
GO:0030532 [small nuclear ribonucleoprotein complex]
GO:0030619 [U1 snRNA binding]
GO:0030620 [U2 snRNA binding]
GO:0030623 [U5 snRNA binding]
GO:0044822 [poly(A) RNA binding]
GO:0070530 [K63-linked polyubiquitin binding]
GO:0071013 [catalytic step 2 spliceosome]
GO:0071222 [cellular response to lipopolysaccharide]
GO:0071356 [cellular response to tumor necrosis factor]
GO:0097157 [pre-mRNA intronic binding]
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 2335 aa
273.6 kDa 		No 0
PRPF8-003
ENSP00000458151
ENST00000577001
I3L0J9 [Direct mapping]
Pre-mRNA-processing-splicing factor 8
Show all
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000386 [second spliceosomal transesterification activity]
GO:0000398 [mRNA splicing, via spliceosome]
GO:0003676 [nucleic acid binding]
GO:0003723 [RNA binding]
GO:0005681 [spliceosomal complex]
GO:0017070 [U6 snRNA binding]
GO:0030619 [U1 snRNA binding]
GO:0030620 [U2 snRNA binding]
GO:0030623 [U5 snRNA binding]
GO:0097157 [pre-mRNA intronic binding]
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 1013 aa
119.8 kDa 		No 0
PRPF8-013
ENSP00000460849
ENST00000573725
I3L3Z8 [Direct mapping]
Pre-mRNA-processing-splicing factor 8
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Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000375 [RNA splicing, via transesterification reactions]
GO:0000386 [second spliceosomal transesterification activity]
GO:0017070 [U6 snRNA binding]
GO:0030619 [U1 snRNA binding]
GO:0030620 [U2 snRNA binding]
GO:0030623 [U5 snRNA binding]
GO:0097157 [pre-mRNA intronic binding]
Show all
 250 aa
29.4 kDa 		No 0
PRPF8-017
ENSP00000459095
ENST00000571958
I3L1T8 [Direct mapping]
Pre-mRNA-processing-splicing factor 8
Show all
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Cancer-related genes
   Mutational cancer driver genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000375 [RNA splicing, via transesterification reactions]
GO:0000386 [second spliceosomal transesterification activity]
GO:0017070 [U6 snRNA binding]
GO:0030619 [U1 snRNA binding]
GO:0030620 [U2 snRNA binding]
GO:0030623 [U5 snRNA binding]
GO:0097157 [pre-mRNA intronic binding]
Show all
 116 aa
12.4 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.