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LIG4

GENERAL INFORMATION

Gene name

 LIG4

Gene description

 Ligase IV, DNA, ATP-dependent

Protein class

 Cancer-related genes
Disease related genes
Enzymes
Potential drug targets
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 5
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 Mainly nuclear expression in all tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly consistent with RNA expression data.

Reliability score

 Supported based on 1 antibody.
HPA001334
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PROGNOSTIC SUMMARY
Gene product is not prognostic.
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY
HPA001334

GENE INFORMATION

Gene name

 LIG4

Synonyms

Description

 Ligase IV, DNA, ATP-dependent (HGNC Symbol)

Entrez gene summary

 The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Chromosome

 13

Cytoband

 q33.3

Chromosome location (bp)

 108207439 - 108218368

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000174405 (version 83.38)

Entrez gene

 3981

UniProt

 P49917 (UniProt - Evidence at protein level)

neXtProt

 NX_P49917

Antibodypedia

 LIG4 antibodies

PROTEIN BROWSER
LIG4-001
LIG4-002
LIG4-003
LIG4-004
LIG4-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
LIG4-001
ENSP00000484288
ENST00000611712
P49917 [Direct mapping]
DNA ligase 4
A0A024RE06 [Target identity:100%; Query identity:100%]
DNA ligase
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Potential drug targets
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000012 [single strand break repair]
GO:0000793 [condensed chromosome]
GO:0001701 [in utero embryonic development]
GO:0002328 [pro-B cell differentiation]
GO:0003677 [DNA binding]
GO:0003909 [DNA ligase activity]
GO:0003910 [DNA ligase (ATP) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0005958 [DNA-dependent protein kinase-DNA ligase 4 complex]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0007049 [cell cycle]
GO:0007417 [central nervous system development]
GO:0008022 [protein C-terminus binding]
GO:0008283 [cell proliferation]
GO:0010165 [response to X-ray]
GO:0010212 [response to ionizing radiation]
GO:0010332 [response to gamma radiation]
GO:0016032 [viral process]
GO:0016874 [ligase activity]
GO:0032807 [DNA ligase IV complex]
GO:0033077 [T cell differentiation in thymus]
GO:0033151 [V(D)J recombination]
GO:0033152 [immunoglobulin V(D)J recombination]
GO:0033153 [T cell receptor V(D)J recombination]
GO:0035019 [somatic stem cell population maintenance]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045190 [isotype switching]
GO:0046872 [metal ion binding]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050769 [positive regulation of neurogenesis]
GO:0051102 [DNA ligation involved in DNA recombination]
GO:0051103 [DNA ligation involved in DNA repair]
GO:0051276 [chromosome organization]
GO:0051301 [cell division]
GO:0051402 [neuron apoptotic process]
GO:0070419 [nonhomologous end joining complex]
GO:0071285 [cellular response to lithium ion]
GO:0071897 [DNA biosynthetic process]
GO:0075713 [establishment of integrated proviral latency]
GO:0097680 [double-strand break repair via classical nonhomologous end joining]
Show all
 911 aa
104 kDa 		No 0
LIG4-002
ENSP00000385955
ENST00000405925
P49917 [Direct mapping]
DNA ligase 4
A0A024RE06 [Target identity:100%; Query identity:100%]
DNA ligase
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000012 [single strand break repair]
GO:0000793 [condensed chromosome]
GO:0001701 [in utero embryonic development]
GO:0002328 [pro-B cell differentiation]
GO:0003677 [DNA binding]
GO:0003909 [DNA ligase activity]
GO:0003910 [DNA ligase (ATP) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0005958 [DNA-dependent protein kinase-DNA ligase 4 complex]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0007049 [cell cycle]
GO:0007417 [central nervous system development]
GO:0008022 [protein C-terminus binding]
GO:0008283 [cell proliferation]
GO:0010165 [response to X-ray]
GO:0010332 [response to gamma radiation]
GO:0016032 [viral process]
GO:0016874 [ligase activity]
GO:0032807 [DNA ligase IV complex]
GO:0033077 [T cell differentiation in thymus]
GO:0033151 [V(D)J recombination]
GO:0033152 [immunoglobulin V(D)J recombination]
GO:0033153 [T cell receptor V(D)J recombination]
GO:0035019 [somatic stem cell population maintenance]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045190 [isotype switching]
GO:0046872 [metal ion binding]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050769 [positive regulation of neurogenesis]
GO:0051102 [DNA ligation involved in DNA recombination]
GO:0051103 [DNA ligation involved in DNA repair]
GO:0051276 [chromosome organization]
GO:0051301 [cell division]
GO:0051402 [neuron apoptotic process]
GO:0070419 [nonhomologous end joining complex]
GO:0071897 [DNA biosynthetic process]
GO:0075713 [establishment of integrated proviral latency]
GO:0097680 [double-strand break repair via classical nonhomologous end joining]
Show all
 911 aa
104 kDa 		No 0
LIG4-003
ENSP00000402030
ENST00000442234
P49917 [Direct mapping]
DNA ligase 4
A0A024RE06 [Target identity:100%; Query identity:100%]
DNA ligase
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000012 [single strand break repair]
GO:0000793 [condensed chromosome]
GO:0001701 [in utero embryonic development]
GO:0002328 [pro-B cell differentiation]
GO:0003677 [DNA binding]
GO:0003909 [DNA ligase activity]
GO:0003910 [DNA ligase (ATP) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0005958 [DNA-dependent protein kinase-DNA ligase 4 complex]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0007049 [cell cycle]
GO:0007417 [central nervous system development]
GO:0008022 [protein C-terminus binding]
GO:0008283 [cell proliferation]
GO:0010165 [response to X-ray]
GO:0010332 [response to gamma radiation]
GO:0016032 [viral process]
GO:0016874 [ligase activity]
GO:0032807 [DNA ligase IV complex]
GO:0033077 [T cell differentiation in thymus]
GO:0033151 [V(D)J recombination]
GO:0033152 [immunoglobulin V(D)J recombination]
GO:0033153 [T cell receptor V(D)J recombination]
GO:0035019 [somatic stem cell population maintenance]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045190 [isotype switching]
GO:0046872 [metal ion binding]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050769 [positive regulation of neurogenesis]
GO:0051102 [DNA ligation involved in DNA recombination]
GO:0051103 [DNA ligation involved in DNA repair]
GO:0051276 [chromosome organization]
GO:0051301 [cell division]
GO:0051402 [neuron apoptotic process]
GO:0070419 [nonhomologous end joining complex]
GO:0071897 [DNA biosynthetic process]
GO:0075713 [establishment of integrated proviral latency]
GO:0097680 [double-strand break repair via classical nonhomologous end joining]
Show all
 911 aa
104 kDa 		No 0
LIG4-004
ENSP00000480814
ENST00000614526
A0A0C4DGV9 [Direct mapping]
DNA ligase
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003677 [DNA binding]
GO:0003909 [DNA ligase activity]
GO:0003910 [DNA ligase (ATP) activity]
GO:0005524 [ATP binding]
GO:0006281 [DNA repair]
GO:0006310 [DNA recombination]
GO:0033152 [immunoglobulin V(D)J recombination]
GO:0051103 [DNA ligation involved in DNA repair]
GO:0071897 [DNA biosynthetic process]
Show all
 844 aa
96.2 kDa 		No 0
LIG4-201
ENSP00000349393
ENST00000356922
P49917 [Direct mapping]
DNA ligase 4
A0A024RE06 [Target identity:100%; Query identity:100%]
DNA ligase
Show all
Enzymes
   ENZYME proteins
   Ligase
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
Potential drug targets
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000012 [single strand break repair]
GO:0000793 [condensed chromosome]
GO:0001701 [in utero embryonic development]
GO:0002328 [pro-B cell differentiation]
GO:0003677 [DNA binding]
GO:0003909 [DNA ligase activity]
GO:0003910 [DNA ligase (ATP) activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0005925 [focal adhesion]
GO:0005958 [DNA-dependent protein kinase-DNA ligase 4 complex]
GO:0006266 [DNA ligation]
GO:0006281 [DNA repair]
GO:0006297 [nucleotide-excision repair, DNA gap filling]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006310 [DNA recombination]
GO:0007049 [cell cycle]
GO:0007417 [central nervous system development]
GO:0008022 [protein C-terminus binding]
GO:0008283 [cell proliferation]
GO:0010165 [response to X-ray]
GO:0010332 [response to gamma radiation]
GO:0016032 [viral process]
GO:0016874 [ligase activity]
GO:0032807 [DNA ligase IV complex]
GO:0033077 [T cell differentiation in thymus]
GO:0033151 [V(D)J recombination]
GO:0033152 [immunoglobulin V(D)J recombination]
GO:0033153 [T cell receptor V(D)J recombination]
GO:0035019 [somatic stem cell population maintenance]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045190 [isotype switching]
GO:0046872 [metal ion binding]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0050769 [positive regulation of neurogenesis]
GO:0051102 [DNA ligation involved in DNA recombination]
GO:0051103 [DNA ligation involved in DNA repair]
GO:0051276 [chromosome organization]
GO:0051301 [cell division]
GO:0051402 [neuron apoptotic process]
GO:0070419 [nonhomologous end joining complex]
GO:0071897 [DNA biosynthetic process]
GO:0075713 [establishment of integrated proviral latency]
GO:0097680 [double-strand break repair via classical nonhomologous end joining]
Show all
 911 aa
104 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.