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Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus.
Antibody dilution
1:34
1:143
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019585
Antibody HPA065603
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human lung shows strong membranous positivity in alveolar cells.
Expression
RNA: detected in 35 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019585
Antibody HPA065603
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414203)
Target mass (kDa)
23.6, 23.5, 16.3, 16.2
31.1, 23.6, 23.5, 19
Antibody dilution
1:250
1:480
PROTEIN ARRAY
Antibody HPA019585
Antibody HPA065603
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.