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DDIT3

ANTIBODY INFORMATION
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA058416   HPA068416  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 14   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA058416, RRID:AB_2683712   Atlas Antibodies Cat#HPA068416, RRID:AB_2685985  

VALIDATION SUMMARY
 
 

Antibody HPA058416

  

Antibody HPA068416

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:161    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Standard validation

   Approved  

   

Figure description

   Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 65 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1250  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 21.7, 19.2, 19.1, 16   21.7, 19.2, 19.1, 16  

Antibody dilution

 1:540   1:640  

PROTEIN ARRAY
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6450   1:7700  

ANTIGEN INFORMATION
 
 

Antibody HPA058416

  

Antibody HPA068416

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 76   76  

Antigen sequence

 
AESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIF
TTLDPASLAWLTEEEPEPAEVTSTSQ
  
AESLPFSFGTLSSWELEAWYEDLQEVLSSDENGGTYVSPPGNEEEESKIF
TTLDPASLAWLTEEEPEPAEVTSTSQ
 

Matching transcripts

 DDIT3-001 - ENSP00000340671 [100%]
DDIT3-002 - ENSP00000448665 [100%]
DDIT3-004 - ENSP00000447503 [100%]
DDIT3-005 - ENSP00000447803 [100%]
DDIT3-006 - ENSP00000447188 [100%]
DDIT3-201 - ENSP00000481270 [95%]
  DDIT3-001 - ENSP00000340671 [100%]
DDIT3-002 - ENSP00000448665 [100%]
DDIT3-004 - ENSP00000447503 [100%]
DDIT3-005 - ENSP00000447803 [100%]
DDIT3-006 - ENSP00000447188 [100%]
DDIT3-201 - ENSP00000481270 [95%]
 

Other gene match

    
ANTIGEN VIEW
DDIT3-001
DDIT3-002
DDIT3-004
DDIT3-005
DDIT3-006
DDIT3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.