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Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum & the Golgi apparatus.
Immunofluorescent staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum.
Antibody dilution
1:32
1:51
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039468
Antibody HPA048119
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows distinct positivity in endothelial cells as well as glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 25 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039468
Antibody HPA048119
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406300)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406300)
Target mass (kDa)
51.6
51.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039468
Antibody HPA048119
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
