We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Expression
RNA: detected in 1 tissues Protein: detected in 39 cell types
RNA: detected in 1 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:65
1:1500
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046141
Antibody HPA050600
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406567)
Target mass (kDa)
38.5
38.5
Antibody dilution
1:125
1:1830
PROTEIN ARRAY
Antibody HPA046141
Antibody HPA050600
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
