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Immunofluorescent staining of human cell line BJ shows localization to nucleoplasm.
Antibody dilution
1:78
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA050317
Standard validation
Approved
Figure description
Immunohistochemical staining of human uterus, pre-menopause shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 26 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA050317
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404286)
Target mass (kDa)
25.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA050317
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
