RNA category is based on mRNA expression levels in the analyzed samples (RNA assay description). The categories include: tissue/cell line enriched, group enriched, tissue/cell line enhanced, expressed in all, mixed and not detected. RNA category is calculated separately for The Cancer Genome Atlas (TCGA) data from cancer tissues and internally generated Human Protein Atlas (HPA) data from normal tissues and cell lines.
TCGA (cancer tissue):
Expressed in all
HPA (cell line):
Cell line enhanced (RH-30)
HPA (normal tissue):
Expressed in all
Protein evidence scores are generated from several independent sources and are classified as evidence at i) protein level, ii) transcript level, iii) no evidence, or iv) not available.
Evidence at protein level
Protein expression normal tissuei
A summary of the overall protein expression pattern across the analyzed normal tissues. The summary is based on knowledge-based annotation.
"Estimation of protein expression could not be performed. View primary data." is shown for genes analyzed with a knowledge-based approach where available RNA-seq and gene/protein characterization data has been evaluated as not sufficient in combination with immunohistochemistry data to yield a reliable estimation of the protein expression profile.
Nuclear expression in several tissues, mostly in a fraction of the cells.
ANTIBODY IHC RELIABILITY
Data reliability descriptioni
Standardized explanatory sentences with additional information required for full understanding of the knowledge-based expression profile.
Antibody staining mainly consistent with RNA expression data.
Reliability score (score description), divided into Supported, Approved, or Uncertain, is evaluated in normal tissues and based on consistency between the staining pattern of one antibody or several antibodies with RNA-seq data and available gene/protein characterization data.
Kaplan-Meier plots for all cancers where high expression of this gene has significant (p<0.001) association with patient survival are shown in this summary. Whether the prognosis is favourable or unfavourable is indicated in brackets. Each Kaplan-Meier plot is clickable and redirects to a detailed page that includes individual expression and survival data for patients with the selected cancer.
Gene product is not prognostic.
RNA EXPRESSION OVERVIEWi
RNA expression overview shows RNA-seq data from The Cancer Genome Atlas (TCGA).
RNA-seq data in 17 cancer types are reported as median FPKM (number Fragments Per Kilobase of exon per Million reads), generated by the The Cancer Genome Atlas (TCGA). RNA cancer tissue category is calculated based on mRNA expression levels across all 17 cancer tissues and include: cancer tissue enriched, cancer group enriched, cancer tissue enhanced, expressed in all, mixed and not detected. To access cancer specific RNA and prognostic data, click on the cancer name.
Antibody staining in 20 different cancers is summarized by a selection of four standard cancer tissue samples representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional fifth image can be added as a complement. The assay and annotation is described here. Note that samples used for immunohistochemistry by the Human Protein Atlas do not correspond to samples in the TCGA dataset.
For each cancer, the fraction of samples with protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the left). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The mouse-over function displays additional data for the features in the staining summary view.
Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.
A manually written summary of the overall protein expression pattern across the analyzed cancer tissues.
Moderate to strong nuclear immunoreactivity was observed in squamous cell carcinomas. Most other tumors were negative.
Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.
JUN (HGNC Symbol)
Jun proto-oncogene (HGNC Symbol)
Entrez gene summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.
At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.
Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).
If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.
Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.
The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.
The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.
The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.
The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.
Predicted intracellular proteins Transcription factors Basic domains Cancer-related genes Candidate cancer biomarkers COSMIC somatic mutations in cancer genes COSMIC Amplifications COSMIC Somatic Mutations FDA approved drug targets Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014)
GO:0000228 [nuclear chromosome] GO:0000790 [nuclear chromatin] GO:0000978 [RNA polymerase II core promoter proximal region sequence-specific DNA binding] GO:0000980 [RNA polymerase II distal enhancer sequence-specific DNA binding] GO:0000981 [RNA polymerase II transcription factor activity, sequence-specific DNA binding] GO:0001077 [transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding] GO:0001102 [RNA polymerase II activating transcription factor binding] GO:0001190 [transcriptional activator activity, RNA polymerase II transcription factor binding] GO:0001525 [angiogenesis] GO:0001774 [microglial cell activation] GO:0001836 [release of cytochrome c from mitochondria] GO:0001889 [liver development] GO:0001938 [positive regulation of endothelial cell proliferation] GO:0002224 [toll-like receptor signaling pathway] GO:0002755 [MyD88-dependent toll-like receptor signaling pathway] GO:0002756 [MyD88-independent toll-like receptor signaling pathway] GO:0003151 [outflow tract morphogenesis] GO:0003677 [DNA binding] GO:0003682 [chromatin binding] GO:0003690 [double-stranded DNA binding] GO:0003700 [transcription factor activity, sequence-specific DNA binding] GO:0003705 [transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding] GO:0003713 [transcription coactivator activity] GO:0005096 [GTPase activator activity] GO:0005515 [protein binding] GO:0005634 [nucleus] GO:0005654 [nucleoplasm] GO:0005667 [transcription factor complex] GO:0005719 [nuclear euchromatin] GO:0005829 [cytosol] GO:0006351 [transcription, DNA-templated] GO:0006355 [regulation of transcription, DNA-templated] GO:0007179 [transforming growth factor beta receptor signaling pathway] GO:0007184 [SMAD protein import into nucleus] GO:0007568 [aging] GO:0007612 [learning] GO:0007623 [circadian rhythm] GO:0008134 [transcription factor binding] GO:0008284 [positive regulation of cell proliferation] GO:0008285 [negative regulation of cell proliferation] GO:0009314 [response to radiation] GO:0009612 [response to mechanical stimulus] GO:0009987 [cellular process] GO:0010033 [response to organic substance] GO:0010634 [positive regulation of epithelial cell migration] GO:0010941 [regulation of cell death] GO:0014070 [response to organic cyclic compound] GO:0017053 [transcriptional repressor complex] GO:0019899 [enzyme binding] GO:0030224 [monocyte differentiation] GO:0031103 [axon regeneration] GO:0031953 [negative regulation of protein autophosphorylation] GO:0032496 [response to lipopolysaccharide] GO:0032870 [cellular response to hormone stimulus] GO:0033613 [activating transcription factor binding] GO:0034097 [response to cytokine] GO:0034134 [toll-like receptor 2 signaling pathway] GO:0034138 [toll-like receptor 3 signaling pathway] GO:0034142 [toll-like receptor 4 signaling pathway] GO:0034146 [toll-like receptor 5 signaling pathway] GO:0034162 [toll-like receptor 9 signaling pathway] GO:0034166 [toll-like receptor 10 signaling pathway] GO:0035026 [leading edge cell differentiation] GO:0035497 [cAMP response element binding] GO:0035666 [TRIF-dependent toll-like receptor signaling pathway] GO:0035994 [response to muscle stretch] GO:0038095 [Fc-epsilon receptor signaling pathway] GO:0038123 [toll-like receptor TLR1:TLR2 signaling pathway] GO:0038124 [toll-like receptor TLR6:TLR2 signaling pathway] GO:0042127 [regulation of cell proliferation] GO:0042493 [response to drug] GO:0042542 [response to hydrogen peroxide] GO:0043065 [positive regulation of apoptotic process] GO:0043066 [negative regulation of apoptotic process] GO:0043392 [negative regulation of DNA binding] GO:0043524 [negative regulation of neuron apoptotic process] GO:0043525 [positive regulation of neuron apoptotic process] GO:0043547 [positive regulation of GTPase activity] GO:0043565 [sequence-specific DNA binding] GO:0043922 [negative regulation by host of viral transcription] GO:0043923 [positive regulation by host of viral transcription] GO:0044212 [transcription regulatory region DNA binding] GO:0044822 [poly(A) RNA binding] GO:0045087 [innate immune response] GO:0045597 [positive regulation of cell differentiation] GO:0045657 [positive regulation of monocyte differentiation] GO:0045740 [positive regulation of DNA replication] GO:0045892 [negative regulation of transcription, DNA-templated] GO:0045893 [positive regulation of transcription, DNA-templated] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0048146 [positive regulation of fibroblast proliferation] GO:0048661 [positive regulation of smooth muscle cell proliferation] GO:0051090 [regulation of sequence-specific DNA binding transcription factor activity] GO:0051365 [cellular response to potassium ion starvation] GO:0051403 [stress-activated MAPK cascade] GO:0051591 [response to cAMP] GO:0051726 [regulation of cell cycle] GO:0051899 [membrane depolarization] GO:0060395 [SMAD protein signal transduction] GO:0061029 [eyelid development in camera-type eye] GO:0070374 [positive regulation of ERK1 and ERK2 cascade] GO:0070412 [R-SMAD binding] GO:0071277 [cellular response to calcium ion] GO:0071837 [HMG box domain binding] GO:1902895 [positive regulation of pri-miRNA transcription from RNA polymerase II promoter] GO:1990441 [negative regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress] GO:2000144 [positive regulation of DNA-templated transcription, initiation]