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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoli & vesicles.
Antibody dilution
1:42
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA052759
Antibody HPA060522
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human prostate shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052759
Antibody HPA060522
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
21.1
21.1
Antibody dilution
1:200
1:140
PROTEIN ARRAY
Antibody HPA052759
Antibody HPA060522
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1600
1:1700
ANTIGEN INFORMATION
Antibody HPA052759
Antibody HPA060522
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
29
51
Antigen sequence
VATIRDSKEKIKASSQIATPLRASRLTRH
ERPTDATVIPSAKRERKAITLDLKLEVLRRFEAGEKLSQIAKALDLAIST
V
