Fields »
ABOUT
HELP
BLOG
PLEC

ANTIBODY INFORMATION
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Epitomics
  

Product name

 HPA025967   HPA029906   1399-1  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 6   6   2  

References

      

Proper citation

 Atlas Antibodies Cat#HPA025967, RRID:AB_10601671   Atlas Antibodies Cat#HPA029906, RRID:AB_10602279    

VALIDATION SUMMARY
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA029906.		  
Validated

Antibody staining overlaps with antibody HPA025967.		    
 

Standard validation

 Approved   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & intermediate filaments.   Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol, intermediate filaments & focal adhesion sites.    

Antibody dilution

 1:50   1:200    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 
   

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA029906 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA025967 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:325   1:250  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		  

     

Figure description

      

Target mass (kDa)

 531.8, 518.5, 518, 516.5, 516.3, 516.2, 514.8, 513.7, 512.6   531.8, 518.5, 518, 516.5, 516.3, 516.2, 514.8, 513.7, 512.6   531.8, 518.5, 518, 516.5, 516.3, 516.2, 514.8, 513.7, 512.6, 80.8, 23.8, 22.5, 19.7, 9.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA025967

  

Antibody HPA029906

  

Antibody CAB003847

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 87   101    

Antigen sequence

 
LTGLSLLPLSEKAARARQEELYSELQARETFEKTPVEVPVGGFKGRTVTV
WELISSEYFTAEQRQELLRQFRTGKVTVEKVIKILIT
  
TEIIRQQGLASYDYVRRRLTAEDLFEARIISLETYNLLREGTRSLREALE
AESAWCYLYGTGSVAGVYLPGSRQTLSIYQALKKGLLSAEVARLLLEAQA
A
  

											 
																				

																		

										
											
Matching transcripts

												
																					PLEC-001 - ENSP00000323856 [100%] 
PLEC-002 - ENSP00000388180 [100%] 
PLEC-003 - ENSP00000434583 [100%] 
PLEC-004 - ENSP00000344848 [100%] 
PLEC-005 - ENSP00000346602 [100%] 
PLEC-006 - ENSP00000350277 [100%] 
PLEC-007 - ENSP00000381756 [100%] 
PLEC-008 - ENSP00000347044 [100%] 
PLEC-009 - ENSP00000348702 [100%] 

											 
																						PLEC-001 - ENSP00000323856 [100%] 
PLEC-002 - ENSP00000388180 [100%] 
PLEC-003 - ENSP00000434583 [100%] 
PLEC-004 - ENSP00000344848 [100%] 
PLEC-005 - ENSP00000346602 [100%] 
PLEC-006 - ENSP00000350277 [100%] 
PLEC-007 - ENSP00000381756 [100%] 
PLEC-008 - ENSP00000347044 [100%] 
PLEC-009 - ENSP00000348702 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PLEC-001
PLEC-002
PLEC-003
PLEC-004
PLEC-005
PLEC-006
PLEC-007
PLEC-008
PLEC-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.