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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:20
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023609
Antibody HPA024619
Antibody HPA025717
Standard validation
Uncertain
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human tonsil shows moderate positivity in reaction center cells while cells outside the reaction center were strongly positive.
Immunohistochemical staining of human cerebellum shows strong dot like nucleolar and cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subsets of seminiferous duct cells.
Expression
RNA: detected in 1 tissues Protein: detected in 21 cell types
RNA: detected in 1 tissues Protein: detected in 53 cell types
RNA: detected in 1 tissues Protein: detected in 58 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:300
1:500
1:125
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023609
Antibody HPA024619
Antibody HPA025717
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
69.8
69.8
69.8
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA023609
Antibody HPA024619
Antibody HPA025717
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.