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ANTIBODY INFORMATION
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   GeneTex
   Novus Biologicals
  

Product name

 HPA002242   GTX30255   NB600-101  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Not known   Not known  

Other gene match

      

Released in version

 3   1   5  

References

      

Proper citation

 Atlas Antibodies Cat#HPA002242, RRID:AB_1078384     Novus Cat#NB600-101, RRID:AB_2069792  

VALIDATION SUMMARY
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  
Immunohistochemistry


Validated
   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		      

     

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.      
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity.   Immunohistochemical staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:300   1:300  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 48.1   48.1, 28.4, 19   48.1, 28.4, 19  

Antibody dilution

 1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA002242

  

Antibody CAB001513

  

Antibody CAB019952

  

Antigen

 Recombinant protein fragment   Not known   Not known  

Length (aa)

 132      

Antigen sequence

 
EQNIDCGGGYVKLFPNSLDQTDMHGDSEYNIMFGPDICGPGTKKVHVIFN
YKGKNVLINKDIRCKDDEFTHLYTLIVRPDNTYEVKIDNSQVESGSLEDD
WDFLPPKKIKDPDASKPEDWDERAKIDDPTDS
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CALR-001 - ENSP00000320866 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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