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Antibody staining overlaps with antibody HPA059398.
Validated
Antibody staining overlaps with antibody HPA030730.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Antibody dilution
1:93
1:137
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030730
Antibody HPA046672
Antibody HPA059398
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes with distinctly stained kupffer cells.
Expression
RNA: detected in 32 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030730
Antibody HPA046672
Antibody HPA059398
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.7
35.7
35.7, 7.2, 6.1
Antibody dilution
1:250
1:390
1:460
PROTEIN ARRAY
Antibody HPA030730
Antibody HPA046672
Antibody HPA059398
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3100
1:5500
ANTIGEN INFORMATION
Antibody HPA030730
Antibody HPA046672
Antibody HPA059398
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
51
39
Antigen sequence
RFRLSSHFIRHRRAHMRRRLYICAGCGRDFKLPPGATAATATERCPECEG
S
RFRLSSHFIRHRRAHMRRRLYICAGCGRDFKLPPGATAATATERCPECEG
S
