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IDH2

ANTIBODY INFORMATION
 
 

Antibody HPA007831

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA007831  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 5  

References

 1  

Proper citation

 Atlas Antibodies Cat#HPA007831, RRID:AB_1851397  

VALIDATION SUMMARY
 
 

Antibody HPA007831

  
Immunocytochemistry

Supported  
Immunohistochemistry

Supported  
Western blot

Supported  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007831

  

Standard validation

 Supported  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:32  

Literature conformity

 The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007831

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007831

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

 

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 50.9, 45.2  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA007831

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA007831

  

Antigen

 Recombinant protein fragment  

Length (aa)

 131  

Antigen sequence

 
REPIICKNIPRLVPGWTKPITIGRHAHGDQYKATDFVADRAGTFKMVFTP
KDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSCFQYAIQKKWPLYM
STKNTILKAYDGRFKDIFQEIFDKHYKTDFD
 

Matching transcripts

 IDH2-001 - ENSP00000331897 [100%]
IDH2-004 - ENSP00000446147 [100%]
 

Other gene match

  
ANTIGEN VIEW
IDH2-001
IDH2-004

RELEVANT PUBLICATIONS
 
 

Antibody HPA007831

  

1.

 Single muscle fiber proteomics reveals unexpected mitochondrial specialization
Murgia M et al
EMBO Rep 2015;16(3):387-95
Application: IHC 		 
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.