We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line BJ shows localization to nucleus.
Antibody dilution
1:30
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018852
Antibody CAB016722
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human prostate shows distinct positivity in plasma.
Plasma shows distinct positivity.
Expression
RNA: detected in 37 tissues Protein: detected in 24 cell types
RNA: detected in 37 tissues Protein: detected in 0 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:15
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018852
Antibody CAB016722
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400655)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
76.7, 75.9, 57.5
76.7, 75.9, 57.5, 19.3, 17.4, 11.8, 11.5
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA018852
Antibody CAB016722
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
