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AP2A2

ANTIBODY INFORMATION
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA043040   sc-55497  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 13   4  

References

    

Proper citation

 Atlas Antibodies Cat#HPA043040, RRID:AB_2678283   Santa Cruz Biotechnology Cat#sc-55497, RRID:AB_2056344  

VALIDATION SUMMARY
 
 

Antibody HPA043040

  

Antibody CAB017514

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry

  Supported  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.   Immunofluorescent staining of human cell line A-431 shows localization to cell junctions & vesicles.  

Antibody dilution

 1:54   1:200  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.  

Expression

   RNA: detected in 36 tissues
Protein: detected in 71 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:1000  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 104.1, 104   104.1, 104, 42, 20.8, 20.5, 15.6, 13.9, 9.8, 6.9, 6.7  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA043040

  

Antibody CAB017514

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 32    

Antigen sequence

 
SGLLVDVFSDSASVVAPLAPGSEDNFARFVCK
  

											 
																				

																		

										
											
Matching transcripts

												
																					AP2A2-001 - ENSP00000413234 [100%] 
AP2A2-201 - ENSP00000327694 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AP2A2-001
AP2A2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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