Cell atlas - BRF1 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	BRF1
Gene description	BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Protein class	Disease related genes Predicted intracellular proteins Predicted secreted proteins
Predicted localization	Intracellular,Secreted
Number of transcripts	16
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nucleoplasm. In addition localized to the nuclear bodies.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (approved)
Additional location	In addition localized to the Nuclear bodies (approved)
Single-cell variation	Single-cell variation in protein expression observed.
Mouse	Localized to the Nucleoplasm in mouse cells.

DATA RELIABILITY
Reliability score	Approved based on 1 antibody. HPA051918
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ASSAYS

RNA expression

Human cells

Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nucleoplasm. In addition localized to the nuclear bodies.

Main location

Nucleoplasm (approved)

Additional location

Nuclear bodies (approved)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location	Single-cell variation
HPA051918	MCF7	Nucleoplasm
HPA051918	MCF7	Nuclear bodies
HPA051918	PC-3	Nucleoplasm
HPA051918	PC-3	Nuclear bodies	Intensity
HPA051918	U-2 OS	No staining

MOUSE CELLS

Main location

Nucleoplasm

Mouse gene

ENSMUSG00000011158 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA051918	NIH 3T3	Nucleoplasm

GENE INFORMATION
Gene name	BRF1 (HGNC Symbol)
Synonyms	BRF, GTF3B, hBRF, TAF3B2, TAF3C, TFIIIB90
Description	BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (HGNC Symbol)
Entrez gene summary	This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]
Chromosome	14
Cytoband	q32.33
Chromosome location (bp)	105209286 - 105315589
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000185024 (version 83.38)
Entrez gene	2972
UniProt	Q92994 (UniProt - Evidence at protein level)
neXtProt	NX_Q92994
Antibodypedia	BRF1 antibodies

PROTEIN BROWSER

BRF1-001	BRF1-002	BRF1-007	BRF1-008	BRF1-009	BRF1-010	BRF1-011	BRF1-012	BRF1-015	BRF1-016	BRF1-017	BRF1-018	BRF1-019	BRF1-022	BRF1-201	BRF1-202

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
BRF1-001 ENSP00000376340 ENST00000392557	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit A0A024R6P8 [Target identity:100%; Query identity:100%] BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae), isoform CRA_b Show all	Phobius predicted secreted proteins Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	473 aa 51.8 kDa	No	0
BRF1-002 ENSP00000448387 ENST00000547530	B3KWA2 [Direct mapping] Transcription factor IIIB 90 kDa subunit; cDNA FLJ42674 fis, clone BRAMY2029988, highly similar to Transcription factor IIIB 90 kDa subunit Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] GO:0043488 [regulation of mRNA stability] GO:0045945 [positive regulation of transcription from RNA polymerase III promoter] Show all	677 aa 73.8 kDa	No	0
BRF1-007 ENSP00000446901 ENST00000551787	Q3SYD7 [Direct mapping] BRF1 protein; Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	161 aa 18.3 kDa	No	0
BRF1-008 ENSP00000388877 ENST00000440513	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	SPOCTOPUS predicted membrane proteins Phobius predicted secreted proteins Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	584 aa 64.3 kDa	No	0
BRF1-009 ENSP00000329029 ENST00000327359	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	SPOCTOPUS predicted membrane proteins Phobius predicted secreted proteins Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	562 aa 61.8 kDa	No	0
BRF1-010 ENSP00000369269 ENST00000379937	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	650 aa 71.1 kDa	No	0
BRF1-011 ENSP00000389859 ENST00000446501	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Predicted intracellular proteins Disease related genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	439 aa 48 kDa	No	0
BRF1-012 ENSP00000446707 ENST00000548421	Q92994 [Direct mapping] Transcription factor IIIB 90 kDa subunit V9HVY2 [Target identity:100%; Query identity:100%] Epididymis secretory sperm binding protein Li 76p Show all	Predicted intracellular proteins Disease related genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0005654 [nucleoplasm] GO:0006383 [transcription from RNA polymerase III promoter] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0009303 [rRNA transcription] GO:0009304 [tRNA transcription] GO:0010467 [gene expression] GO:0017025 [TBP-class protein binding] Show all	208 aa 22.3 kDa	No	0
BRF1-015 ENSP00000448823 ENST00000550692	F8VXJ4 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	64 aa 7.2 kDa	No	0
BRF1-016 ENSP00000450258 ENST00000546417	H0YIV6 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	185 aa 20.9 kDa	No	0
BRF1-017 ENSP00000449788 ENST00000552127	F8VWT8 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	155 aa 17.5 kDa	No	0
BRF1-018 ENSP00000448723 ENST00000549655	F8VS45 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	234 aa 25.6 kDa	No	0
BRF1-019 ENSP00000449173 ENST00000550208	F8VWY1 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Predicted secreted proteins Secreted proteins predicted by MDSEC Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	45 aa 5.2 kDa	Yes	0
BRF1-022 ENSP00000447521 ENST00000550375	F8W123 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		14 aa 1.8 kDa	No	0
BRF1-201 ENSP00000369264 ENST00000379932	F6VUY7 [Direct mapping] Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	134 aa 15.1 kDa	No	0
BRF1-202 ENSP00000480452 ENST00000619151	A3KN61 [Direct mapping] BRF1 protein; Transcription factor IIIB 90 kDa subunit Show all	Phobius predicted secreted proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0000126 [transcription factor TFIIIB complex] GO:0006355 [regulation of transcription, DNA-templated] GO:0006384 [transcription initiation from RNA polymerase III promoter] GO:0008270 [zinc ion binding] GO:0017025 [TBP-class protein binding] Show all	184 aa 20.7 kDa	No	0