Cell atlas - NELFA - The Human Protein Atlas

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NELFA

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TISSUE

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CELL

PATHOLOGY

CELL ATLAS RNA EXPRESSION HUMAN CELLS

GENE/PROTEIN

Antibody validation

Dictionary
	Cytosol
	Nuclear bodies
	Nucleoplasm

Human cell
	Cytosol
	Nucleoplasm

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GENERAL INFORMATION
Gene name	NELFA
Gene description	Negative elongation factor complex member A
Protein class	Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	8
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Mainly localized to the nuclear bodies & nucleoplasm. In addition localized to the cytosol.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Nucleoplasm (validated), Nuclear bodies (validated)
Additional location	In addition localized to the Cytosol (uncertain)

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA039858 , HPA043931
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Mainly localized to the nuclear bodies & nucleoplasm. In addition localized to the cytosol.

Main location

Nucleoplasm (validated), Nuclear bodies (validated)

Additional location

Cytosol (uncertain)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location	Single-cell variation	Cell cycle dependent variation
	HPA039858	CACO-2	Nucleoplasm
	HPA039858	CACO-2	Nuclear bodies
	HPA039858	HEK 293	Nucleoplasm
	HPA039858	HEK 293	Nuclear bodies
	HPA039858	U-2 OS	Nucleoplasm
	HPA039858	U-2 OS	Nuclear bodies
	HPA043931	PC-3	Nucleoplasm
	HPA043931	PC-3	Cytosol
	HPA043931	U-2 OS	Nucleoplasm

GENE INFORMATION
Gene name	NELFA (HGNC Symbol)
Synonyms	NELF-A, WHSC2
Description	Negative elongation factor complex member A (HGNC Symbol)
Entrez gene summary	This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008]
Chromosome	4
Cytoband	p16.3
Chromosome location (bp)	1982714 - 2041903
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000185049 (version 83.38)
Entrez gene	7469
UniProt	Q9H3P2 (UniProt - Evidence at protein level)
neXtProt	NX_Q9H3P2
Antibodypedia	NELFA antibodies

PROTEIN BROWSER

NELFA-001	NELFA-003	NELFA-006	NELFA-010	NELFA-011	NELFA-012	NELFA-015	NELFA-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
NELFA-001 ENSP00000372335 ENST00000382882	A0A0C4DFX9 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0003682 [chromatin binding] GO:0005634 [nucleus] GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] GO:0045944 [positive regulation of transcription from RNA polymerase II promoter] GO:0051571 [positive regulation of histone H3-K4 methylation] Show all	539 aa 58.5 kDa	No	0
NELFA-003 ENSP00000387647 ENST00000416258	H0Y3X6 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] Show all	532 aa 58 kDa	No	0
NELFA-006 ENSP00000395761 ENST00000431323	C9JHL4 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] Show all	294 aa 32.4 kDa	No	0
NELFA-010 ENSP00000396996 ENST00000411649	H7C0W2 [Direct mapping] Negative elongation factor A Show all	SCAMPI predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] Show all	187 aa 20.6 kDa	No	0
NELFA-011 ENSP00000410154 ENST00000455762	C9JEM7 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] Show all	208 aa 22.9 kDa	No	0
NELFA-012 ENSP00000405119 ENST00000453740	H7C2C7 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] Show all	246 aa 26.4 kDa	No	0
NELFA-015 ENSP00000399165 ENST00000411638	Q9H3P2 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Plasma proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] GO:0005654 [nucleoplasm] GO:0005737 [cytoplasm] GO:0006366 [transcription from RNA polymerase II promoter] GO:0006368 [transcription elongation from RNA polymerase II promoter] GO:0007275 [multicellular organismal development] GO:0010467 [gene expression] GO:0016032 [viral process] GO:0032021 [NELF complex] GO:0034244 [negative regulation of transcription elongation from RNA polymerase II promoter] GO:0050434 [positive regulation of viral transcription] Show all	528 aa 57.3 kDa	No	0
NELFA-201 ENSP00000445757 ENST00000542778	A0A0C4DFX9 [Direct mapping] Negative elongation factor A Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0032021 [NELF complex] Show all	539 aa 58.5 kDa	No	0

Contact

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The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.