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CCDC137

ANTIBODY INFORMATION
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052637   HPA053914  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA052637, RRID:AB_2681894   Atlas Antibodies Cat#HPA053914, RRID:AB_2682306  

VALIDATION SUMMARY
 
 

Antibody HPA052637

  

Antibody HPA053914

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA053914.		  
Validated

Antibody staining overlaps with antibody HPA052637.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli.  

Antibody dilution

 1:5   1:93  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human colon shows moderate nuclear positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 40 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:300  

Literature conformity

   No avaliable gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 33.5, 33.2   33.5, 33.2  

Antibody dilution

 1:80   1:460  

PROTEIN ARRAY
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3700  

ANTIGEN INFORMATION
 
 

Antibody HPA052637

  

Antibody HPA053914

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 77   76  

Antigen sequence

 
RSRQEMKNPISNKKRKKAAQVTFRKTLEKEAKGEEPDIAVPKFKQRKGES
DGAYIHRMQQEAQHVLFLSKNQAIRQP
  
QRSVSKDQPGRRSQMLRMLLSPGGVSQPLTASLARQRIVEEERERAVQAY
RALKQRQQQLHGERPHLTSRKKPEPQ
 

Matching transcripts

 CCDC137-001 - ENSP00000329360 [100%]
CCDC137-003 - ENSP00000458350 [90%]
  CCDC137-001 - ENSP00000329360 [100%]
CCDC137-003 - ENSP00000458350 [93%]
 

Other gene match

    
ANTIGEN VIEW
CCDC137-001
CCDC137-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.