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Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
Antibody dilution
1:72
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008398
Antibody HPA008533
Standard validation
Supported
Figure description
Immunohistochemical staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Expression
RNA: detected in 28 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
Antibody dilution
1:400
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008398
Antibody HPA008533
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405823)
Target mass (kDa)
14.3
14.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA008398
Antibody HPA008533
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA008398
Antibody HPA008533
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
51
Antigen sequence
VLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPET
E
VLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPET
E