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Immunofluorescent staining of human cell line MCF7 shows localization to nucleoli.
Antibody dilution
1:8
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011324
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human tonsil shows strong nuclear positivity in reaction center cells and lymphoid cells outside reaction centra.
Expression
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
Antibody dilution
1:10
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011324
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415504)
Target mass (kDa)
24.4
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA011324
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:500
ANTIGEN INFORMATION
Antibody HPA011324
Antigen
Recombinant protein fragment
Length (aa)
101
Antigen sequence
LGKLGMQPGRQHSIFGDPKKIVTEEFVRRGYLIYKPVPRSSPVEYEFFWG
PRAHVESSKLKVMHFVARVRNRCSKDWPCNYDWDSDDDAEVEAILNSGAR
G
