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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:167
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018821
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows strong granular positivity in the cytoplasm of exocrine pancreatic glands, islet cells were weakly stained.
Expression
RNA: detected in 30 tissues Protein: detected in 73 cell types
Retrieval
HIER pH6
Antibody dilution
1:75
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018821
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
50.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA018821
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
