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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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H2AFX
  • premium

    TISSUE

  • premium

    CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

H2AFX

Gene description

H2A histone family, member X

Protein class

Plasma proteins
Predicted intracellular proteins

Predicted localization

Intracellular

Number of transcripts

1
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Ubiquitous nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Caution, targets protein from more than one gene. Correlation with RNA expression data cannot be evaluated due to technical limitations (poly-A tail missing).

Reliability score

Supported based on 2 antibodies.
HPA041189 , CAB012264
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable) and liver cancer (unfavourable).
Renal cancer p<0.001
Liver cancer p<0.001
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA041189
CAB012264

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells showed weak to moderate nuclear staining. Gliomas along with occasional cases of breast and urothelial cancers showed strong positivity.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong nuclear positivity was observed in cancer cells.

GENE INFORMATION

Gene name

H2AFX (HGNC Symbol)

Synonyms

H2AX

Description

H2A histone family, member X (HGNC Symbol)

Entrez gene summary

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Chromosome

11

Cytoband

q23.3

Chromosome location (bp)

119093854 - 119095467

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000188486 (version 83.38)

Entrez gene

3014

UniProt

P16104 (UniProt - Evidence at protein level)

neXtProt

NX_P16104

Antibodypedia

H2AFX antibodies

PROTEIN BROWSER

H2AFX-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

H2AFX-001
ENSP00000434024
ENST00000530167
P16104 [Direct mapping]
Histone H2AX
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   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Plasma proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000077 [DNA damage checkpoint]
GO:0000724 [double-strand break repair via homologous recombination]
GO:0000781 [chromosome, telomeric region]
GO:0000785 [chromatin]
GO:0000786 [nucleosome]
GO:0000790 [nuclear chromatin]
GO:0000794 [condensed nuclear chromosome]
GO:0001673 [male germ cell nucleus]
GO:0001741 [XY body]
GO:0003677 [DNA binding]
GO:0003684 [damaged DNA binding]
GO:0005515 [protein binding]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005657 [replication fork]
GO:0005694 [chromosome]
GO:0006281 [DNA repair]
GO:0006302 [double-strand break repair]
GO:0006303 [double-strand break repair via nonhomologous end joining]
GO:0006325 [chromatin organization]
GO:0006334 [nucleosome assembly]
GO:0006342 [chromatin silencing]
GO:0006974 [cellular response to DNA damage stimulus]
GO:0007283 [spermatogenesis]
GO:0010212 [response to ionizing radiation]
GO:0016032 [viral process]
GO:0019899 [enzyme binding]
GO:0021987 [cerebral cortex development]
GO:0035861 [site of double-strand break]
GO:0042393 [histone binding]
GO:0045739 [positive regulation of DNA repair]
GO:0046982 [protein heterodimerization activity]
GO:0051321 [meiotic cell cycle]
GO:0070062 [extracellular exosome]
GO:0071480 [cellular response to gamma radiation]
GO:0090398 [cellular senescence]
Show all
143 aa
15.1 kDa
No 0

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